Topic > Essay on Photosynthetic Pigments - 1526

Data from the chromatographic part of the experiment showed that the least polar of the pigments would travel the most and the most polar would travel the least; chlorophyll b was the most polar and carotene the least polar. The spectrophotometric part of our experiment also supports this, showing us which wavelengths the pigments reflect and absorb. In every experiment, however, there are sources of error. A source of error in this experiment could include failure to clean the cuvettes before inserting them into the spectrophotometer. The smallest fingerprints or particles can lead to an inaccurate transmittance reading. Additionally, failure to use the reference cuvette when changing the wavelength is a source of error because it will lead to an inaccurate reading. Sources of error when using chromatography paper include too much or too little time required for the solvent to rise onto the paper and the possibility that the solvent level may be too high. When studying wavelengths and photosynthetic pigments, it is known that, depending on the plant, some pigments are absorbed during photosynthesis while others are not. Pigments absorb only the light energy needed to carry out photosynthesis. This knowledge can help determine which areas