ILab report of general biology 3Laboratory n. 18: Gel Electrophoresis Expiration Date: 11/21/13 Name: Jaimin Maknojia Purpose: The purpose of this lab is to analyze the results of the current criminal investigation. Introduction: Gel electrophoresis is used to separate molecules such as RNA, DNA, and proteins . DNA fragments are separated based on size, and proteins are separated based on size and their charge. Gel electrophoresis uses positive and negative electrodes to separate charged particles. Charged particles are attracted to opposite charges (Purdue University 2012). Since DNA has a negative charge, the positively charged electrical circuit is placed at the bottom of the gel. This causes smaller DNA particles to move rapidly through the gel (Brooker 2012). In this experiment, 3 DNA samples are provided. DNA from the blood left at the scene (crime scene DNA). And the DNA from the swabs taken from the cheeks of both suspects (suspect A and suspect B). This DNA was administered to test two different markers (Marker 1 and Marker 2) and to find out which DNA has the same profile. The gel is made of algae. It is a gel made up of small wells at the end of the gel (Biotechnology Learning Hub). STR stands for short tandem repeats. DNA samples are created from 2 versions of the genome. One is inherited from the mother and the other is inherited from the father. These genomes are then used to compare DNA samples.Materials:Prepackaged agarose gelGel boxMan pipettesPipette tipsDNA Solutions: Crime Scene Markers 1 and 2Suspect A Markers 1 and 2Suspect B Markers 1 and TBE BufferCarbon Fiber ElectrodesAliment .. .... half of the sheet ...... and marker matching, the next step in a forensic investigation would be to test the remaining 11 markers for a complete DNA profile. DNA fingerprinting can be used to find out who the child's parents are, if they are in the situation where a child is swapped. It can also be used to find out who the real father or mother is, if either of them has had an affair with someone other than their spouse. DNA fingerprinting also allows any adopted child to find out who his or her biological parents are (Rafael 2009). References: Brooker, Robert J., Eric Widmaier, Linda Graham and Peter Stiling. (2011). Genetic technology. Biology, 415.Purdue University Van Project. (1996). Retrieved December 1, 2012. from http://www.chem.purdue.edu/te Rafael. The benefits of DNA fingerprinting. Retrieved August 22, 2009. http://www.brighthub.com/science/genetics/articles/46432.aspx.